Animated Titania

Not so fast my friend! She appeared very briefly in a quick fight scene in an earlier episode*, she growled, Shulkie punched her, and the fight was over (not really, but it was switching between all the Hulks, and the others got more fight time.) But I think this is her first dialogue appearance.

*listed as episode 12, "Into the Negative Zone"

Wasn't she also in the animated Sensational She Hulk series of the early 80's. I could be wrong, and frequently am.

Not to my knowledge. I know she was created in Marvel's Secret Wars, which was in the early 80's (83? 84?) but even there she didn't get much screen time, just an introduction and her first contact with She Hulk and Crusher Creel. and afterwards, I think was listed as a Spider-Man villain, and appeared occasionally with Crusher. I think she did not appear in the Hulk cartoon in the 90's either. Heck Jen didn't until the second season, and then it was more like the adventures of Jen and Bruce.

Ok, that was from my noggin, someone fact check me against the internets!
Titania entry on Comic Vine